Biotin BromoCatch™ Ligand
Tocris Bioscience, part of Bio-Techne | Catalog # 8939
A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
Key Product Details
Description
A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
Product Description
Biotin BromoCatch™ Ligand is a covalent probe designed for the selective labeling and enrichment of proteins tagged with the BromoCatch™ self-labeling tag. Based on a para-acrylamide-functionalized bump-JQ1 scaffold, the BromoCatch™ ligand enables irreversible and highly specific covalent attachment to the engineered cysteine residue in the BromoCatch-tagged protein. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. This probe demonstrates exceptional target selectivity, confirmed via intact protein mass spectrometry and western blotting, and shows no background labeling in untransfected or wild-type samples even at micromolar concentrations. Biotin BromoCatch™ Ligand expands the utility of the BromoCatch™ platform for biochemical workflows including proteomics, immunoprecipitation, and interaction studies.Please refer to this protocol for further information on how to use this product.
This product is part of an early adopter program.
BromoCatch™ is a trademark of Bio-Techne Corp.
Scientific Data Images for Biotin BromoCatch™ Ligand
Biotin probe for cellular affinity-based pull-down applications.
Cell lysate experiments show that Biotin BromoCatch™ Ligand specifically detects H2B-BromoCatch at 0.25 µM concentration of probe.
Biotin affinity pull-down mass spectrometry analysis in a transiently transfected BromoCatch-GFP HEK293 line.
Parental or BromoCatch-GFP expressing HEK293 cells were treated with Biotin BromoCatch™ Ligand (1 μM), and probe-interacting proteins were pulled down using streptavidin-coated beads. Mass spectrometry protein enrichment analysis demonstrates specificity of BromoCatch™ Biotin Ligand and ability to pull down the BromoCatch-GFP construct. Licensing Information
Sold under exclusive licence from the University of DundeeProduct Specifications
Molecular Weight
908.15
Formula
C44H61N9O8S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-((17R)-17-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-16-oxo-3,6,9,12-tetraoxa-15-azanonadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
InChI Key
BZQKBFKRTBEEFV-HQANLXDBSA-N
SMILES
CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCOCCNC(CCCC[C@H]4[C@](NC(N5)=O)([H])[C@]5([H])CS4)=O)=O)CC)C6=NN=C(C)N26
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Rodriguez-Rios BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. Nat. Commun. 2026 PMID: 42128888
Product Specific Notices for Biotin BromoCatch™ Ligand
For research use only
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