BCI

Name: BCI
Brand: Tocris Bioscience
SKU: 7481

Tocris Bioscience, part of Bio-Techne | Catalog # 7481

Citations (1)

COA / SDS

DUSP6 and DUSP1 inhibitor

Key Product Details

Description:	DUSP6 and DUSP1 inhibitor
Chemical Name:	3-(Cyclohexylamino)-2,3-dihydro-2-(phenylmethylene)-1H-inden-1-one
Purity:	≥98% (HPLC)
Molecular Weight:	317.42

View Full Technical Details for BCI

Technical Data
Citations

Biological Activity for BCI

BCI is an allosteric inhibitor of dual specificity phosphatase DUSP6 and DUSP1 (EC₅₀ values are 8.0 μM and 13.3 μM respectively). Increases P2X₇ receptor expression in neuroblastoma cells, reduces cell viability of EGFR or KRAS mutant lung cancer cells, and induces apoptosis in p53-null non-small cell lung cancer cells. In an animal model of myocardial infarction, BCI improves heart function, reduces fibrosis, and reduces inflammation by inhibiting macrophage formation. Activates FGF signaling in zebrafish embryo development to increase myocardial progenitor population and heart size.

Technical Data for BCI

M. Wt	317.42
Formula	C₂₂H₂₃NO
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	15982-84-0
PubChem ID	6419844
InChi Key	XJDKPLZUXCIMIS-HMMYKYKNSA-N
Smiles	O=C1/C(C(C2=C1C=CC=C2)NC3CCCCC3)=C/C4=CC=CC=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BCI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	31.74	100
	ethanol	15.87	50

Preparing Stock Solutions for BCI

The following data is based on the product molecular weight 317.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.15 mL	15.75 mL	31.50 mL
5 mM	0.63 mL	3.15 mL	6.30 mL
10 mM	0.32 mL	1.58 mL	3.15 mL
50 mM	0.06 mL	0.32 mL	0.63 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for BCI

References are publications that support the biological activity of the product.

Unni Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells. eLife 2018 PMID: 30475204
Shin BCI induces apoptosis via generation of reactive oxygen species and activation of intrinsic mitochondrial pathway in H1299 lung cancer cells. Sci.China Life Sci. 2018 PMID: 29524123
Benito-León BCI, an inhibitor of the DUSP1 and DUSP6 dual specificity phosphatases, enhances P2X7 receptor expression in neuroblastoma cells. Front.Cell Dev.Biol. 2022 PMID: 36589747
Zhang A DUSP6 inhibitor suppresses inflammatory cardiac remodeling and improves heart function after myocardial infarction. Dis.Model.Mech. 2023 PMID: 36478044

BCI