AM 404

Tocris Bioscience, part of Bio-Techne | Catalog # 1116

COA / SDS

Anandamide transport inhibitor

Key Product Details

Description:	Anandamide transport inhibitor
Chemical Name:	N-(4-Hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide
Purity:	≥98% (HPLC)
Molecular Weight:	395.58

View Full Technical Details for AM 404

Technical Data
Citations

Biological Activity for AM 404

AM 404 is a competitive and selective inhibitor of carrier-mediated anandamide transport (IC₅₀ = 1 μM). Does not activate CB₁ receptors or inhibit anandamide hydrolysis but has been shown to activate native and cloned vanilloid receptors (pEC₅₀ = 7.4). Also FABP inhibitor. Active in vivo.

Technical Data for AM 404

M. Wt	395.58
Formula	C₂₆H₃₇NO₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	183718-77-6
PubChem ID	6604822
InChi Key	IJBZOOZRAXHERC-DOFZRALJSA-N
Smiles	CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)NC1=CC=C(O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AM 404

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	19.78	50
	DMSO	19.78	50

Preparing Stock Solutions for AM 404

The following data is based on the product molecular weight 395.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.06 mL	25.28 mL	50.56 mL
2.5 mM	1.01 mL	5.06 mL	10.11 mL
5 mM	0.51 mL	2.53 mL	5.06 mL
25 mM	0.10 mL	0.51 mL	1.01 mL

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Reconstitution Calculator

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References for AM 404

References are publications that support the biological activity of the product.

Deutsch A personal retrospective: Elevating Anandamide (AEA) by targeting fatty acid amide hydrolase (FAAH) and the fatty acid binding proteins (FABPs). Front.Pharmacol. 2017 PMID: 27790143
Zygmunt The anandamide transport inhibitor AM404 activates vanilloid receptors. Eur.J.Pharmacol. 2000 PMID: 10822052
Calignano Potentiation of anandamide hypotension by the transport inhibitor, AM 404. Eur.J.Pharmacol. 1997 PMID: 9389389
Piomelli Structural determinants for recognition and translocation by the anandamide transporter. Proc.Natl.Acad.Sci.U.S.A. 1999 PMID: 10318965
Beltramo Functional role of high-affinity anandamide transport, as revealed by selective inhibition. Science 1997 PMID: 9262477