Ac4ManNDAz
Tocris Bioscience, part of Bio-Techne | Catalog # 7553
Photo-crosslinking probe for studying glycoproteins and glycolipids
Key Product Details
Description
Photo-crosslinking probe for studying glycoproteins and glycolipids
Product Description
Ac4ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac4ManNDAz is metabolized and incorporated into cell surface glycoproteins as a photo-crosslinking sialic acid analog. Upon UV irradiation, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby molecules.Product Specifications
Molecular Weight
457.44
Formula
C19H27N3O10
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2-Deoxy-2-[[3-(3-methyl-3H-diazirin-3-yl)-1-oxopropyl]amino]-D-mannopyranose-1,3,4,6-tetraacetate
CAS Number
1015698-39-1
PubChem ID
122400676
InChI Key
UZYLHDJBFVVKBM-GAMQSXCYSA-N
SMILES
CC(OC[C@H]1OC([C@H]([C@H]([C@@H]1OC(C)=O)OC(C)=O)NC(CCC2(N=N2)C)=O)OC(C)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.74 | 100 | |
| Ethanol | 45.74 | 100 |
Preparing Stock Solutions
for Ac4ManNDAz
The following data is based on the product molecular weight 457.44
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.19 mL | 10.93 mL | 21.86 mL |
| 5 mM | 0.44 mL | 2.19 mL | 4.37 mL |
| 10 mM | 0.22 mL | 1.09 mL | 2.19 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Cervin GM1 ganglioside-independent intoxication by Cholera toxin. PLoS.Pathog. 2018 PMID: 29432456
- Bond Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars. Nat.Protoc. 2009 PMID: 19536272
- Bond Metabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides. Bioconjug.Chem. 2011 PMID: 21838313
- Tanaka Photoactivatable crosslinking sugars for capturing glycoprotein interactions. J.Am.Chem.Soc. 2008 PMID: 18293988
Product Specific Notices for Ac4ManNDAz
For research use only
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