A23187, free acid is a calcium ionophore that induces Ca²⁺-dependent cell death by increasing intracellular calcium concentration. Promotes intracelllular ROS generation and platelet particle formation (fragmentation) in vitro and in vivo. Can be used to induce autophagy in mammalian cells.

Product Specifications

Molecular Weight

523.63

Formula

C₂₉H₃₇N₃O₆

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11-trimethyl-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7-dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid

CAS Number

52665-69-7

PubChem ID

11957499

InChI Key

HIYAVKIYRIFSCZ-CYEMHPAKSA-N

SMILES

[H][C@@]1([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@@H](C)[C@@]2(O[C@H](CC3=NC(C(C(O)=O)=C(NC)C=C4)=C4O3)[C@H](C)CC2)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.62	5

Preparing Stock Solutions for A23187, free acid

The following data is based on the product molecular weight 523.63

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	38.19 mL	190.97 mL	381.95 mL
0.25 mM	7.64 mL	38.19 mL	76.39 mL
0.5 mM	3.82 mL	19.10 mL	38.19 mL
2.5 mM	0.76 mL	3.82 mL	7.64 mL

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Background References

References are publications that support the biological activity of the product.

Ding Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 2007 PMID: 17135238
Nardi Platelet particle formation by anti-GPIIIa49-66 Ab, Ca2+ ionophore A23187, and phorbol myristate acetate is induced by reactive oxygen species and inhibited by dexameth. blockade of platelet phospholipase A2, 12-lipoxygenase, and Blood 2007 PMID: 17545506
Sakanashi Zn²⁺, derived from cell preparation, partly attenuates Ca²⁺-dependent cell death induced by A23187, calcium ionophore, in rat thymocytes. Toxicol.In Vitro 2009 PMID: 19124067
Kajitani Mechanism of A23187-induced apoptosis in HL-60 cells: dependency on mitochondrial permeability transition but not NADPH oxidase. Biosci.Biotechnol.Bichem. 2007 PMID: 7535265

A23187, free acid

Tocris Bioscience, part of Bio-Techne | Catalog # 1234

Key Product Details

Description

Alternative Names

View all Calcium Signaling Agents »

Product Description