A 33

Name: A 33
Brand: Tocris Bioscience
SKU: 6313

Tocris Bioscience, part of Bio-Techne | Catalog # 6313

Citations (6)

COA / SDS

Potent and selective PDE4B inhibitor

Key Product Details

Description:	Potent and selective PDE4B inhibitor
Chemical Name:	2-[4-[[2-(5-Chloro-2-thienyl)-5-ethyl-6-methyl-pyrimidin-4-yl]amino]phenyl]acetic acid
Purity:	≥98% (HPLC)
Molecular Weight:	387.88

View Full Technical Details for A 33

Technical Data
Citations

Biological Activity for A 33

A 33 is a potent and selective PDE4B inhibitor (IC₅₀ = 15 nM); exhibits >100-fold selectivity for PDE4B over 4D. Regulates cAMP in inflammatory cells. Inhibits LPS-induced TNF-α production and neutrophil accumulation in mouse lungs. Reduces microglial and neutrophil activation, and improves behavioral deficits in a traumatic brain injury model in vivo. Also exhibits antidepressant-like effects. Orally bioavailable.

Technical Data for A 33

M. Wt	387.88
Formula	C₁₉H₁₈ClN₃O₂S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	915082-52-9
PubChem ID	15951010
InChi Key	FDVSPBLZPJMXFV-UHFFFAOYSA-N
Smiles	CCC1=C(N=C(C2=CC=C(S2)Cl)N=C1NC3=CC=C(C=C3)CC(O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for A 33

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	38.79	100

Preparing Stock Solutions for A 33

The following data is based on the product molecular weight 387.88.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.58 mL	12.89 mL	25.78 mL
5 mM	0.52 mL	2.58 mL	5.16 mL
10 mM	0.26 mL	1.29 mL	2.58 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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References for A 33

References are publications that support the biological activity of the product.

Zhang Comparison of the pharmacological profiles of selective PDE4B and PDE4D inhibitors in the central nervous system. Sci.Rep. 2017 PMID: 28054669
Wilson Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury. PLoS One 2017 PMID: 28542295
Naganuma Discovery of selective PDE4B inhibitors. Bioorg.Med.Chem.Lett. 2009 PMID: 19447034