5J 4

Name: 5J 4
Brand: Tocris Bioscience
SKU: 5490

Tocris Bioscience, part of Bio-Techne | Catalog # 5490

Citations (1)

COA / SDS

CRAC channel blocker; anti-inflammatory

Key Product Details

Description:	CRAC channel blocker; anti-inflammatory
Chemical Name:	N-[[(6-Hydroxy-1-naphthalenyl)amino]thioxomethyl]-2-furancarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	312.34

View Full Technical Details for 5J 4

Technical Data
Citations

Biological Activity for 5J 4

5J 4 is a calcium release-activated calcium (CRAC) channel blocker. Blocks store-operated calcium entry (SOCE) in HeLa-O+S cells and Th17 cells in vitro. Ameliorates symptoms and reduces CNS infiltration by mononuclear cells in a mouse model of experimental autoimmune encephalomyelitis (EAE) in vivo.

Technical Data for 5J 4

M. Wt	312.34
Formula	C₁₆H₁₂N₂O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	827001-82-1
PubChem ID	2968289
InChi Key	WMUSJLJASFXGHN-UHFFFAOYSA-N
Smiles	OC1=CC2=C(C=C1)C(NC(NC(C3=CC=CO3)=O)=S)=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5J 4

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	31.23	100
	ethanol	3.12	10

Preparing Stock Solutions for 5J 4

The following data is based on the product molecular weight 312.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.20 mL	16.01 mL	32.02 mL
5 mM	0.64 mL	3.20 mL	6.40 mL
10 mM	0.32 mL	1.60 mL	3.20 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

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References for 5J 4

References are publications that support the biological activity of the product.

Kim Calcium signaling via Orai1 is essential for induction of the nuclear orphan receptor pathway to drive Th17 differentiation. J.Immunol. 2014 PMID: 24307733