25-Hydroxycholesterol
Tocris Bioscience, part of Bio-Techne | Catalog # 5741
Inhibits hepatitis C viral life cycle; also α-crystallin (cryAB) chaperone
Key Product Details
Description
Inhibits hepatitis C viral life cycle; also α-crystallin (cryAB) chaperone
Product Description
25-Hydroxycholesterol is an antiviral. Stimulates expression of miR-185 microRNA which inhibits the hepatitis C virus life cycle via inhibition of cholesterol biosynthesis and virus cell membrane fusion. Also α-crystallin (cryAB) chaperone (Kd = 10.1 μM). Blocks α-crystallin aggregation and partially reverses cataract formation by binding and stabilizing soluble forms of cryAB in vitro and in vivo.Product Specifications
Molecular Weight
402.65
Formula
C27H46O2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
Cholest-5-ene-3β,25-diol
CAS Number
2140-46-7
PubChem ID
65094
InChI Key
INBGSXNNRGWLJU-ZHHJOTBYSA-N
SMILES
O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| Ethanol | 8.05 | 20 |
Preparing Stock Solutions
for 25-Hydroxycholesterol
The following data is based on the product molecular weight 402.65
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 12.42 mL | 62.09 mL | 124.18 mL |
| 1 mM | 2.48 mL | 12.42 mL | 24.84 mL |
| 2 mM | 1.24 mL | 6.21 mL | 12.42 mL |
| 10 mM | 0.25 mL | 1.24 mL | 2.48 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Makley Pharmacological chaperone for alpha-crystallin partially restores transparency in cataract models. Science 2015 PMID: 26542570
- Singaravelu MicroRNAs regulate the immunometabolic response to viral infection in the liver. Nat.Chem.Biol. 2015 PMID: 26479438
Product Specific Notices for 25-Hydroxycholesterol
For research use only
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