1-Azakenpaullone New
Tocris Bioscience, part of Bio-Techne | Catalog # 3673
Potent and selective GSK3 inhibitor. Used in chemical cocktail for induction and maintainance of TotiSCs
Key Product Details
Description
Potent and selective GSK3 inhibitor. Used in chemical cocktail for induction and maintainance of TotiSCs
Alternative Names
1-Akp
Product Description
1-Azakenpaullone is a potent and selective glycogen synthase kinase 3 (GSK-3) inhibitor (IC50 = 18 nM). It displays selectivity index (SI) of 111 and 233 over CDK1 and CDK5, respectively. 1-Azakenpaullone stimulates growth and replication of β-cells in vitro (rat islets) and alleviates glucolipotoxicity in INS-1 β-cells. It displays mitogenic effects on rat β-cells comparable with those of incretin hormone GIP. In combination with TTNPB (Cat. No. 0761) and WS 6 (Cat. No. 7791), 1-Azakenpaullone can be part of a chemical cocktail used for induction and long-term maintainance in vitro of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs).Product Specifications
Molecular Weight
328.17
Formula
C15H10BrN3O
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
9-Bromo-7,12-dihydropyridoazepino[4,5-b]indol-6(5H)-one
CAS Number
676596-65-9
PubChem ID
6538897
InChI Key
NTSBZVCEIVPKBJ-UHFFFAOYSA-N
SMILES
BrC2=CC=C1NC3=C(CC(NC4=C3N=CC=C4)=O)C1=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.82 | 100 |
Preparing Stock Solutions
for 1-Azakenpaullone
The following data is based on the product molecular weight 328.17
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.05 mL | 15.24 mL | 30.47 mL |
| 5 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 10 mM | 0.30 mL | 1.52 mL | 3.05 mL |
| 50 mM | 0.06 mL | 0.30 mL | 0.61 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Mussmann Inhibition of GSK3 promotes replication and survival of pancreatic beta cells. J.Biol.Chem. 2007 PMID: 17242403
- Hu Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature 2023 PMID: 35728625
- Kunick 1-Azakenpaullone is a selective inhibitor of glycogen synthase kinase-3β. Bioorg.Med.Chem.Lett. 2004 PMID: 14698171
Product Specific Notices for 1-Azakenpaullone
For research use only
Loading...
Loading...