Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
ProteinSimple, part of Bio-Techne | Catalog # CBS403
Compatible with Peggy Sue and NanoPro 1000 systems
Key Product Details
Features
Kit includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer.
Product Benefits
- Bicine/CHAPS Lysis Buffer contains 20 mM Bicine (pH 7.5), 0.6% CHAPS and 1X DMSO and aqueous inhibitor mixes.
- Aqueous Protease Inhibitor contains 40mM NaF, 12 mM beta-glycerophosphate, 1 mM Sodium Orthovanadate, 0.5mM EDTA, 2.5 mM EGTA
- DMSO Inhibitor Mix contains 1mM AEBSF, 5µg/ml Aprotinin, 50 µM Bestatin, 5µg/ml E-64 Protease Inhibitor, 10 µg/ml Leupeptin, 7µg/mL Pepstatin A, Phosphatase inhibitor cocktail (with effective concentrations of Cantharidin, Bromotetramisole and Microcystin LR)
- Cell Wash Buffer contains 20 mM Bicine (pH 7.5), 250 mM Sucrose
Compatible Instruments
NanoPro 1000 - Simple Western charge-based assays | ProteinSimple
NanoPro 1000 from ProteinSimple, a Bio-Techne brand, characterizes post-translational protein modifications. See beyond what's detected by a traditional Western Blot.
Peggy Sue - Automated Western Blot System | ProteinSimple
Peggy Sue from ProteinSimple, a Bio-Techne brand, automates western blotting technology. Run 96 data points in one overnight run with as little as 5 µL of sample.
Preparation and Storage
Shipping
The product is shipped in multiple packages at different temperatures.
Storage
Please see the package insert for the recommended storage of each component.
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