Simple Plex Non-Human Primate Neurogranin Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-015849

Simple Plex Non-Human Primate Neurogranin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer. Sample type indicated by NP (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

4.23 pg/mL

Assay Range

16.4-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Non-Human Primate Neurogranin Cartridge Summary

Simple Plex assay is for the detection of non-human primate Neurogranin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Neurogranin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Non-human Primate

Specificity

Natural and recombinant Neurogranin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Neurogranin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Neurogranin control were tested in multiple assays performed by at least three technicians using two lots of reagents for non-human primate Neurogranin.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 26 26
Mean (pg/mL) 45.0 2206 35.3 1619
Standard Deviation 2.69 162 4.57 189
CV% 6.0 7.3 12.9 11.7

Recovery for Simple Plex Non-Human Primate Neurogranin Cartridge

Recovery for the non-human primate Neurogranin assays was evaluated at three different spiked concentrations of Neurogranin protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 86 82-90
EDTA Plasma (n=5) 95 91-98
Heparin Plasma (n=4) 97 87-112
Serum (n=4) 92 76-123

Linearity

Samples containing and/or spiked with high concentrations of Neurogranin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Non-Human Primate Neurogranin Assay Linearity

Scientific Data Images for Simple Plex Non-Human Primate Neurogranin Cartridge

Simple Plex Non-Human Primate Neurogranin Assay Standard Curve

Simple Plex Non-Human Primate Neurogranin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate Neurogranin Assay. Assay range is 16.4-4000 pg/mL.
Simple Plex Non-Human Primate Neurogranin Assay

Simple Plex Non-Human Primate Neurogranin Assay

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neurogranin

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. (provided by RefSeq)

Alternate Names

hng, NRGN, RC3

Entrez Gene IDs

4900 (Human); 64011 (Mouse); 64356 (Rat)

Gene Symbol

NRGN

Additional Neurogranin Products

Product Documents for Simple Plex Non-Human Primate Neurogranin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Non-Human Primate Neurogranin Cartridge

For research use only

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