Simple Plex Neurodegeneration Duo Panel – Ultra-Sensitive

Name: Simple Plex Neurodegeneration Duo Panel – Ultra-Sensitive
Brand: ProteinSimple
SKU: SPCKC-PS-015256

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-015256

COA

Simple Plex 32x2 panel containing GFAP 2nd Gen and NF-L with sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).

Key Product Details

Features:

The Simple Plex Human Multianalyte Panel, Neurodegeneration Duo Panel – Ultra-Sensitive, is for the detection of Ultra-Sensitive human GFAP 2nd Gen and NF-L in serum and plasma (EDTA/Heparin).

Sample Type & Volume Required Per Well	Cell Culture Supernates (40 uL), Serum (40 uL), EDTA Plasma (40 uL), Heparin Plasma (40 uL), Cerebrospinal Fluid (40 uL)
Sensitivity	GFAP 2nd Gen 0.19 pg/mL; NF-L 0.42 pg/mL
Assay Range	GFAP 2nd Gen 0.62 – 5,908 pg/mL; NF-L 1.99 – 7,600 pg/mL

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Technical Data
Citations

Summary for Simple Plex Neurodegeneration Duo Panel – Ultra-Sensitive

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human GFAP 2nd Gen and NF-L.
Cross-reactivity
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
GFAP 2nd Gen	n	16	16	72	73
	Mean (pg/mL)	46.1	2063	45.8	1923
	Standard Deviation	2.263	94.8	5.40	171
	CV (%)	4.9	4.6	11.8	8.9
NF-L	n	16	16	83	84
	Mean (pg/mL)	67.0	2873	74.3	3023
	Standard Deviation	1.204	76.5	8.54	246
	CV (%)	1.8	2.7	11.5	8.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
GFAP 2nd Gen	EDTA Plasma (n=5)	82	68-98
	Heparin Plasma (n=4)	99	73-112
	Serum (n=5)	79	66-98
NF-L	EDTA Plasma (n=5)	83	66-97
	Heparin Plasma (n=4)	93	88-99
	Serum (n=4)	87	79-100

Product Specifications for Simple Plex Neurodegeneration Duo Panel – Ultra-Sensitive

Species

Human

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.