Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008305
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VCAM-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each VCAM-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for VCAM-1.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 44 | 43 |
| Mean (pg/mL) | 197 | 9548 | 198 | 10414 |
| Standard Deviation | 8.38 | 546 | 21.2 | 1216 |
| CV% | 4.2 | 5.7 | 10.7 | 11.7 |
Recovery for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
Recovery for the mouse/rat VCAM-1 assays was evaluated at three different spiked concentrations of VCAM-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 105 | 103-107 |
Linearity
Samples containing and/or spiked with high concentrations of mouse/rat VCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
Simple Plex Mouse/Rat VCAM-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat VCAM-1 Assay. Assay range is 7.97-30,372 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: VCAM-1/CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
Long Name
Alternate Names
Gene Symbol
Additional VCAM-1/CD106 Products
Product Documents for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
Product Specific Notices for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
For research use only