Simple Plex Mouse/Rat M-CSF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008920
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat M-CSF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Rat EDTA Plasma, Rat Heparin Plasma, Rat Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 47 | 46 |
| Mean (pg/mL) | 5.74 | 280 | 3.72 | 191 |
| Standard Deviation | 0.39 | 16.6 | 0.33 | 15.5 |
| CV% | 6.9 | 5.9 | 9.0 | 8.1 |
Recovery for Simple Plex Mouse/Rat M-CSF Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 96-100 |
Linearity
Samples containing and/or spiked with high concentrations of mouse/rat M-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat M-CSF Cartridge
Mouse/Rat M-CSF Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: M-CSF
M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
Long Name
Alternate Names
Gene Symbol
Additional M-CSF Products
Product Documents for Simple Plex Mouse/Rat M-CSF Cartridge
Product Specific Notices for Simple Plex Mouse/Rat M-CSF Cartridge
For research use only