Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003998
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-2 R alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-2 R alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse/rat IL-2 R alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 19 | 19 |
| Mean (pg/mL) | 111 | 5388 | 110 | 6115 |
| Standard Deviation | 8.48 | 317 | 7.12 | 629 |
| CV% | 7.7 | 5.9 | 6.5 | 10.3 |
Recovery for Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge
Recovery for the mouse/rat IL-2 R alpha assays was evaluated at three different spiked concentrations of IL-2 R alpha protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Rat Serum (n=4) | 104 | 99-109 |
Linearity
Samples containing and/or spiked with high concentra-tions of mouse/rat IL-2 R alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge
Simple Plex Mouse/Rat IL-2 R alpha Assay Linearity
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat IL-2 R alpha Assay. Assay range is 2.10-20,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CD25/IL-2R alpha
CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2R alpha can be generated by proteolytic cleavage of the cell surface receptor.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional CD25/IL-2R alpha Products
Product Documents for Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge
Product Specific Notices for Simple Plex Mouse/Rat CD25/IL-2 R alpha Cartridge
For research use only