Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Name: Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-000584

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-000584

Simple Plex Mouse/Rat CCL2/JE/MCP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer. Sample type indicated by MP (Plasma/Serum).

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Technical Data
Citations

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (5 µL), Rat Cell Culture Supernates (5 µL), Mouse Serum (25 µL), Rat Serum (5 µL), Mouse EDTA Plasma (25 µL), Rat EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL), Rat Heparin Plasma (25 µL), Mouse Cerebrospinal Fluid (25 µL)

Sensitivity

0.16 pg/mL

Assay Range

0.71-1090 pg/mL (Mouse Cell Culture Supernates, Rat Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Mouse Cerebrospinal Fluid)

Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Monocyte Chemotactic Protein 1 (MCP-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CCL2/JE/MCP-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Mouse, Rat

Specificity

Natural and recombinant mouse/rat MCP-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MCP-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each MCP-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for MCP-1.

Mouse Cell Culture Supernates, Mouse Cerebrospinal Fluid, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Rat Cell Culture Supernates, Rat EDTA Plasma, Rat Heparin Plasma, Rat Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	0	0	0	0
n	16	16	54	54
Mean (pg/mL)	6.41	331	313	6.60
Standard Deviation	0.484	20.2	27.4	0.697
CV%	7.5	6.1	8.8	10.6

Recovery for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Recovery was not necessary as mouse/rat MCP-1 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat MCP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Simple Plex Mouse/Rat MCP-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat MCP-1 Assay. Assay range is 0.71-1090 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4⁺ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

Additional CCL2/JE/MCP-1 Products

Product Documents for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

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Product Specific Notices for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

For research use only

Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge