Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Name: Simple Plex Mouse/Rat Angiopoietin-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-003636

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003636

Simple Plex Mouse/Rat Angiopoietin-2/ANG-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	1.1 pg/mL
Assay Range:	10.5 - 40,130 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse/Rat Angiopoietin-2 Cartridge Summary:	Simple Plex assay is for the detection of mouse and rat Angiopoietin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse and rat Angiopoietin-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Angiopoietin-2 assay

Inter-Assay Precision (Precision between assays) Replicates of each Angiopoietin-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for Angiopoietin-2

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	93	89
Mean (pg/mL)	198	10863	240	11457
Standard Deviation	18.6	452	27.5	1311
CV%	9.4	4.2	11.5	11.4

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Angiopoietin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Rat Angiopoietin-2 Assay Linearity

Scientific Data Examples for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

	Simple Plex Mouse/Rat Angiopoietin-2 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat Angiopoietin-2 Assay. Assay range is 10.5-40,130 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Item	Value
Entrez Gene IDs	285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names	AGPT2, ANG-2, ANG2, ANGPT2, Angiopoietin-2, Tie2-ligand, angiopoietin 2, angiopoietin-2B, angiopoietin-2a, angiopoitin 2

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol