Simple Plex Mouse P-Selectin Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008306
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse P-Selectin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one P-Selectin/CD62P assay.
Inter-Assay Precision (Precision between assays) Replicates of each P-Selectin/CD62P control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse P-Selectin/CD62P.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 53 | 54 |
| Mean (pg/mL) | 220 | 9862 | 199 | 9752 |
| Standard Deviation | 20.4 | 659 | 20.5 | 859 |
| CV% | 9.3 | 6.7 | 10.3 | 8.8 |
Recovery for Simple Plex Mouse P-Selectin Cartridge
Recovery for the mouse P-Selectin/CD62P assays was evaluated at three different spiked concentrations of P-Selectin/CD62P protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 115 | 113-117 |
Linearity
Samples containing and/or spiked with high concentrations of mouse P-Selectin/CD62P were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse P-Selectin Cartridge
Simple Plex Mouse P-Selectin/CD62P Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse P-Selectin/CD62P Assay. Assay range is 2.93-27,879 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: P-Selectin/CD62P
P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Alternate Names
Gene Symbol
Additional P-Selectin/CD62P Products
Product Documents for Simple Plex Mouse P-Selectin Cartridge
Product Specific Notices for Simple Plex Mouse P-Selectin Cartridge
For research use only