Simple Plex Mouse Lipocalin-2 Cartridge

Name: Simple Plex Mouse Lipocalin-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007400

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-007400

Simple Plex Mouse Lipocalin-2/NGAL assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bronchoalveolar Lavage Fluid (10 uL), Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity:	0.9 pg/mL
Assay Range:	4.24 - 2,584 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Mouse Lipocalin-2 Cartridge Summary:	Simple Plex assay is for the detection of mouse Lipocalin-2/NGAL in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and bronchoalveolar lavage fluid (BALF) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Simple Plex Mouse Lipocalin-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse Lipocalin-2/NGAL.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Lipocalin-2/NGAL assay

Inter-Assay Precision (Precision between assays) Replicates of each Lipocalin-2/NGAL control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse Lipocalin-2/NGAL

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	20
Mean (pg/mL)	18.9	928	17.7	918
Standard Deviation	1.36	42.9	1.86	91.7
CV%	7.2	4.6	10.5	10

Linearity

Samples containing and/or spiked with high concentrations of Lipocalin-2/NGAL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse Lipocalin-2 Cartridge

	Simple Plex Mouse Lipocalin-2/NGAL Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse Lipocalin-2/NGAL Assay. Assay range is 4.24-2584 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Item	Value
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

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Product Documents for Simple Plex Mouse Lipocalin-2 Cartridge

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