Simple Plex Mouse GM-CSF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003559
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse GM-CSF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GM-CSF assay.
Inter-Assay Precision (Precision between assays) Replicates of each GM-CSF control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse GM-CSF.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 99 | 98 |
| Mean (pg/mL) | 24.9 | 1518 | 35.9 | 1727 |
| Standard Deviation | 2.07 | 75.6 | 2.97 | 145 |
| CV% | 5.9 | 5.0 | 8.3 | 8.4 |
Recovery for Simple Plex Mouse GM-CSF Cartridge
Recovery for the mouse GM-CSF assays was evaluated at three different spiked concentrations of GM-CSF protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 106 | 101-110 |
| Heparin Plasma (n=4) | 103 | 93-110 |
| Serum (n=4) | 99 | 97-106 |
Linearity
Samples containing and/or spiked with high concentrations of mouse GM-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse GM-CSF Cartridge
Simple Plex Mouse GM-CSF Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse GM-CSF Assay. Assay range is 0.63-6040 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: GM-CSF
GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional GM-CSF Products
Product Documents for Simple Plex Mouse GM-CSF Cartridge
Product Specific Notices for Simple Plex Mouse GM-CSF Cartridge
For research use only