Simple Plex Mouse Erythropoietin/EPO Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009096
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse Erythropoietin/EPO Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 75 | 75 |
| Mean (pg/mL) | 77.0 | 3917 | 91.6 | 4348 |
| Standard Deviation | 2.13 | 96.2 | 10.8 | 363 |
| CV% | 2.8 | 2.5 | 11.8 | 8.3 |
Recovery for Simple Plex Mouse Erythropoietin/EPO Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 103-103 |
| EDTA Plasma (n=4) | 94 | 89-100 |
| Heparin Plasma (n=4) | 83 | 77-90 |
| Serum (n=4) | 100 | 92-106 |
Linearity
Samples containing and/or spiked with high concentrations of mouse Epo were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse Erythropoietin/EPO Cartridge
Mouse Erythropoietin/EPO Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Erythropoietin/EPO
Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.
Alternate Names
Gene Symbol
Additional Erythropoietin/EPO Products
Product Documents for Simple Plex Mouse Erythropoietin/EPO Cartridge
Product Specific Notices for Simple Plex Mouse Erythropoietin/EPO Cartridge
For research use only