Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-000583
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CXCL2/MIP-2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CXCL2/MIP-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse CXCL2/MIP-2.
Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 91 | 92 |
| Mean (pg/mL) | 10.9 | 475 | 10.3 | 463 |
| Standard Deviation | 0.994 | 40.8 | 1.20 | 39.3 |
| CV% | 9.1 | 8.6 | 11.6 | 8.5 |
Recovery for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
Recovery for the mouse CXCL2/MIP-2 assays was evaluated at three different spiked concentrations of CXCL2/MIP-2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse EDTA Plasma (n=4) | 106 | 100-115 |
| Mouse Heparin Plasma (n=4) | 107 | 100-116 |
| Mouse Serum (n=4) | 104 | 94-113 |
Linearity
Samples containing and/or spiked with high concentrations of mouse CXCL2/MIP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
Simple Plex Mouse CXCL2/MIP-2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse CXCL2/MIP-2 Assay. Assay range is 0.92-1412 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CXCL2/GRO beta/MIP-2/CINC-3
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
Alternate Names
Gene Symbol
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
Product Specific Notices for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
For research use only