Simple Plex Mouse C-Reactive Protein/CRP Cartridge

Name: Simple Plex Mouse C-Reactive Protein/CRP Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007804

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-007804

Simple Plex Mouse C-Reactive Protein/CRP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.41 pg/mL
Assay Range:	3.54 - 13,480 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Mouse C-Reactive Protein/CRP Cartridge Summary:	Simple Plex assay for the detection of mouse C-Reactive Protein (CRP) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse C-Reactive Protein/CRP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse C-Reactive Protein/CRP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse CRP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Linearity

Samples containing and/or spiked with high concentrations of mouse CRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse C-Reactive Protein/CRP Cartridge

	Mouse CRP Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Item	Value
Entrez Gene IDs	1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names	C-Reactive Protein, C-reactive protein, pentraxin-related, CRP, MGC88244, PTX1MGC149895, pentraxin 1

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse C-Reactive Protein/CRP Cartridge

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Protocols & Manuals

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