Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009926
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 44 |
| Mean (pg/mL) | 732 | 39604 | 835 | 41220 |
| Standard Deviation | 42.3 | 2036 | 83.6 | 4015 |
| CV% | 5.8 | 5.1 | 10.0 | 9.7 |
Recovery for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 92 | 90-94 |
Linearity
Samples containing and/or spiked with high concentrations of mouse AFP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
Mouse alpha-Fetoprotein/AFP Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: alpha-Fetoprotein/AFP
alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
Additional alpha-Fetoprotein/AFP Products
Product Documents for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
Product Specific Notices for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
For research use only