Simple Plex Human TIMP-1 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001442
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TIMP-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIMP-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each TIMP-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TIMP-1.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 32 | 33 |
| Mean (pg/mL) | 57.8 | 2915 | 54.5 | 2833 |
| Standard Deviation | 4.86 | 152 | 5.97 | 259 |
| CV% | 8.4 | 5.2 | 10.9 | 9.1 |
Recovery for Simple Plex Human TIMP-1 Cartridge
Recovery for the human TIMP-1 assays was evaluated at three different spiked concentrations of TIMP-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 112 | 112-113 |
Linearity
Samples containing and/or spiked with high concentrations of TIMP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human TIMP-1 Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TIMP-1 Assay. Assay range is 1.05-10,000 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: TIMP-1
TIMP-1 (tissue inhibitor of metalloproteinase-1) is a secreted glycoprotein that blocks metalloproteinase (MMP) activity. It circulates in complex with various MMPs to maintain them in an inactive state. It is produced by fibroblasts, osteoblasts, endothelial cells, granulosa cells, dendritic cells, vascular smooth muscle cells, adipocytes, and monocytes. TIMP-1 also protects against apoptosis in B cells, stimulates erythropoiesis and BFU-E expansion, both stimulates and inhibits tumor growth, inhibits angiogenesis, and stimulates gonadal steroidogenesis in conjuction with Cathepsin L.
Long Name
Alternate Names
Gene Symbol
Additional TIMP-1 Products
Product Documents for Simple Plex Human TIMP-1 Cartridge
Product Specific Notices for Simple Plex Human TIMP-1 Cartridge
For research use only