Simple Plex Human S100B Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-009486

Simple Plex Human S100B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer. Sample type indicated with PS (Plasma/Serum).
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ST01C-PS-009486
SPCKA-PS-009486
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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.648 pg/mL

Assay Range

4.01-2448 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Simple Plex Human S100B Cartridge Summary

Simple Plex assay for the detection of human S100 Calcium Binding Protein B (S100B) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF). 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all alpha-Synuclein Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant S100B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 72 71
Mean (pg/mL) 32.0 1845 23.5 1443
Standard Deviation 2.09 67.3 2.16 115
CV% 6.6 3.6 9.2 8.0

Recovery for Simple Plex Human S100B Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 101 93-108
EDTA Plasma (n=4) 96 92-99
Heparin Plasma (n=4) 94 90-98
Saliva (n=3) 89 82-94
Serum (n=4) 94 90-101

Linearity

Samples containing and/or spiked with high concentrations of S100B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human S100B Cartridge

Human S100B Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-Synuclein

The synuclein family includes alpha-Synuclein, beta-Synuclein, gamma-Synuclein, and synoretin. These small, highly conserved proteins are localized predominantly in the brain, concentrated in presynaptic nerve terminals. Alpha-Synuclein is normally a soluble protein. Abnormal alpha-synuclein forms filamentous inclusions (Lewy bodies and Lewy neurites) in several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies, and Alzheimer's disease. Beta-Synuclein and gamma-Synuclein (synoretin) associate with alpha-synuclein and inhibit its aggregation.

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Gene Symbol

SNCA

Additional alpha-Synuclein Products

Product Documents for Simple Plex Human S100B Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human S100B Cartridge

For research use only

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