Simple Plex Human Progranulin/PGRN Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-016248
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Progranulin/PGRN Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Progranulin/PGRN assay.
Inter-Assay Precision (Precision between assays) Replicates of each Progranulin/PGRN control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Progranulin/PGRN.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 49 | 43 |
| Mean (pg/mL) | 1294 | 65001 | 1209 | 62818 |
| Standard Deviation | 66.0 | 1388 | 132 | 4030 |
| CV% | 5.1 | 2.1 | 10.9 | 6.4 |
Recovery for Simple Plex Human Progranulin/PGRN Cartridge
Recovery for the human Progranulin/PGRN assays was evaluated at three different spiked concentrations of Progranulin/PGRN protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 100 | 99-102 |
Linearity
Samples containing and/or spiked with high concentrations of Progranulin/PGRN were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Progranulin/PGRN Cartridge
Simple Plex Human Progranulin/PGRN Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Progranulin/PGRN Assay. Assay range is 21.0-200,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Progranulin/PGRN
Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer's disease, and motor neuron disease.
Alternate Names
Gene Symbol
Additional Progranulin/PGRN Products
Product Documents for Simple Plex Human Progranulin/PGRN Cartridge
Product Specific Notices for Simple Plex Human Progranulin/PGRN Cartridge
For research use only