Simple Plex Human NGFR/TNFRSF16 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000297
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human NGFR/TNFRSF16 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one NGFR assay.
Inter-Assay Precision (Precision between assays) Replicates of each NGFR control were tested in multiple assays performed by at least three technicians using two lots of reagents for human NGFR.
EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 9 | 8 |
| Mean (pg/mL) | 613 | 32,027 | 597 | 35,057 |
| Standard Deviation | 28.5 | 2331 | 39.1 | 2601 |
| CV% | 4.7 | 7.3 | 6.5 | 7.4 |
Recovery for Simple Plex Human NGFR/TNFRSF16 Cartridge
Recovery for the human NGFR assays was evaluated at three different spiked concentrations of NGFR protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 112 | 101-122 |
| Heparin Plasma (n=4) | 106 | 88-119 |
| Serum (n=4) | 108 | 98-115 |
Linearity
Samples containing and/or spiked with high concentrations of human NGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human NGFR/TNFRSF16 Cartridge
Simple Plex Human NGFR Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human NGFR Assay. Assay range is 28.6-108,900 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: NGFR/TNFRSF16
The low affinity nerve growth factor receptor (NGFR), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family1 and has been designated TNFRSF16. The NGF receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGFR also binds several myelin derived proteins (Nogo, myelin associated glycoprotein, and myelin oligodendrocyte glycoprotein) as a co-receptor with Nogo receptor.
The NGF receptor is a 75 kDa protein that is expressed in neuronal axons, Schwanns cells and perineural cells of peripheral nerves. Neural crest stem cells have been isolated based on their surface expression of NGFR. In addition, neuroepithelial-derived NGFR-positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture. The expression of NGFR has also been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells.
Long Name
Alternate Names
Gene Symbol
Additional NGFR/TNFRSF16 Products
Product Documents for Simple Plex Human NGFR/TNFRSF16 Cartridge
Product Specific Notices for Simple Plex Human NGFR/TNFRSF16 Cartridge
For research use only