Simple Plex Human Neutrophil Elastase/ELA2 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011934
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Neutrophil Elastase/ELA2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 78 | 53 |
| Mean (pg/mL) | 63.9 | 4161 | 68.5 | 3515 |
| Standard Deviation | 2.39 | 203 | 8.10 | 325 |
| CV% | 3.7 | 4.9 | 11.9 | 9.3 |
Recovery for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 110 | 109-110 |
Linearity
Samples containing and/or spiked with high concentrations of human ELA2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge
Simple Plex Human Neutophil Elastase/ELA2 Ella Assay
Preparation and Storage
Shipping
Stability & Storage
Background: Neutrophil Elastase/ELA2
Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.
Alternate Names
Gene Symbol
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge
Product Specific Notices for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge
For research use only