Simple Plex Human Insulin Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000507
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Insulin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Insulin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Insulin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Insulin.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 43 | 43 |
| Mean (pmol/L) | 5.93 | 308 | 6.23 | 316 |
| Standard Deviation | 0.359 | 24.5 | 0.864 | 38.3 |
| CV% | 6.1 | 7.9 | 13.9 | 12.1 |
Recovery for Simple Plex Human Insulin Cartridge
Recovery for the human Insulin assays was evaluated at three different spiked concentrations of Insulin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 107 | 103-110 |
Linearity
Samples containing and/or spiked with high concentrations of human Insulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Insulin Cartridge
Simple Plex Human Insulin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Insulin Assay. Assay range is 0.040-400 pmol/L.Preparation and Storage
Shipping
Stability & Storage
Background: Insulin
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. Insulin processes out part of the G, leaving two parallel, but disulfide linked segments; the IGFs only process at the ends, retaining the basic configuration.
Alternate Names
Gene Symbol
Additional Insulin Products
Product Documents for Simple Plex Human Insulin Cartridge
Product Specific Notices for Simple Plex Human Insulin Cartridge
For research use only