Simple Plex Human IL-6 R alpha Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000505
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-6 R alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-6 R alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-6 R alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-6 R alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 46.5 | 2131 | 44.6 | 2219 |
| Standard Deviation | 1.63 | 173 | 1.97 | 85.0 |
| CV% | 3.5 | 8.1 | 4.4 | 3.8 |
Recovery for Simple Plex Human IL-6 R alpha Cartridge
Recovery for the human IL-6 R alpha assays was evaluated at three different spiked concentrations of IL-6 R alpha protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=3) | 97 | 94-101 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-6 R alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-6 R alpha Cartridge
Simple Plex Human IL-6 R alpha Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-6 R alpha Assay. Assay range is 0.78-2976 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-6R alpha
IL-6R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.
Long Name
Alternate Names
Gene Symbol
Additional IL-6R alpha Products
Product Documents for Simple Plex Human IL-6 R alpha Cartridge
Product Specific Notices for Simple Plex Human IL-6 R alpha Cartridge
For research use only