Simple Plex Human IL-33 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000363
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-33 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-33 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-33 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-33.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 12 | 12 |
| Mean (pg/mL) | 6.85 | 326 | 6.67 | 328 |
| Standard Deviation | 0.314 | 17.1 | 0.320 | 13.5 |
| CV% | 4.6 | 5.3 | 4.8 | 4.1 |
Recovery for Simple Plex Human IL-33 Cartridge
Recovery for the human IL-33 assays was evaluated at three different spiked concentrations of IL-33 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 108 | 102-116 |
| Heparin Plasma (n=4) | 106 | 102-112 |
| Serum (n=4) | 110 | 100-121 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-33 Cartridge
Simple Plex Human IL-33 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-33 Assay. Assay range is 0.75-1140 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-33
IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.
Long Name
Alternate Names
Gene Symbol
Additional IL-33 Products
Product Documents for Simple Plex Human IL-33 Cartridge
Product Specific Notices for Simple Plex Human IL-33 Cartridge
For research use only