Simple Plex Human IL-21 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-013133
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-21 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each IL-21 control was tested multiple times in one human IL-21 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-21 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-21.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 52 | 51 |
| Mean (pg/mL) | 18.6 | 952 | 22.5 | 1046 |
| Standard Deviation | 0.964 | 69.2 | 2.42 | 77.0 |
| CV% | 5.2 | 7.3 | 10.8 | 7.4 |
Recovery for Simple Plex Human IL-21 Cartridge
Recovery for the human IL-21 assays was evaluated at three different spiked concentrations of IL-21 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 99 | 97-101 |
| EDTA Plasma (n=4) | 100 | 95-107 |
| Serum (n=4) | 112 | 104-122 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-21 Cartridge
Simple Plex Human IL-21 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-21 Assay. Assay range is 0.78-7,455 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-21
IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
Long Name
Alternate Names
Gene Symbol
Additional IL-21 Products
Product Documents for Simple Plex Human IL-21 Cartridge
Product Specific Notices for Simple Plex Human IL-21 Cartridge
For research use only