Simple Plex Human IL-19 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002815
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-19 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-19 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-19 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-19.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 55.3 | 2740 | 48.9 | 2355 |
| Standard Deviation | 3.10 | 190 | 5.07 | 225 |
| CV% | 5.6 | 6.9 | 10.4 | 9.6 |
Recovery for Simple Plex Human IL-19 Cartridge
Recovery for the human IL-19 assays was evaluated at three different spiked concentrations of IL-19 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 96 | 83-108 |
| Heparin Plasma (n=4) | 96 | 88-106 |
| Serum (n=4) | 97 | 94-99 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-19 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-19 Cartridge
Simple Plex Human IL-19 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-19 Assay. Assay range is 3.07-11,700 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-19
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.
Long Name
Alternate Names
Gene Symbol
Additional IL-19 Products
Product Documents for Simple Plex Human IL-19 Cartridge
Product Specific Notices for Simple Plex Human IL-19 Cartridge
For research use only