Simple Plex Human IL-13 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000499
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-13 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-13 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-13 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-13.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 12 | 12 |
| Mean (pg/mL) | 75.8 | 4104 | 73.5 | 4060 |
| Standard Deviation | 2.01 | 181 | 4.90 | 251 |
| CV% | 2.7 | 4.4 | 6.7 | 6.2 |
Recovery for Simple Plex Human IL-13 Cartridge
Recovery for the human IL-13 assays was evaluated at three different spiked concentrations of IL-13 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 95 | 92-99 |
| EDTA Plasma (n=4) | 103 | 99-112 |
| Heparin Plasma (n=4) | 98 | 93-109 |
| Serum (n=4) | 105 | 96-114 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-13 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-13 Cartridge
Simple Plex Human IL-13 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-13 Assay. Assay range is 3.6-13,720 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: IL-13
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
Long Name
Alternate Names
Gene Symbol
Additional IL-13 Products
Product Documents for Simple Plex Human IL-13 Cartridge
Product Specific Notices for Simple Plex Human IL-13 Cartridge
For research use only