Simple Plex Human IL-10 2nd Gen Assay Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-009385
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-10 2nd Gen Assay Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-10 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-10 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-10.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 35 | 37 |
| Mean (pg/mL) | 8.89 | 462 | 10.8 | 477 |
| Standard Deviation | 0.193 | 16.0 | 0.940 | 24.5 |
| CV% | 2.2 | 3.5 | 8.7 | 5.1 |
Recovery for Simple Plex Human IL-10 2nd Gen Assay Cartridge
Recovery for the human IL-10 assays was evaluated at three different spiked concentrations of IL-10 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 101 | 100-103 |
| EDTA Plasma (n=4) | 91 | 84-93 |
| Heparin Plasma (n=4) | 91 | 79-95 |
| Serum (n=4) | 95 | 93-97 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-10 2nd Gen Assay Cartridge
Simple Plex Human IL-10 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-10 Assay. Assay range is 0.38-1446 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-10
IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-10 Products
Product Documents for Simple Plex Human IL-10 2nd Gen Assay Cartridge
Product Specific Notices for Simple Plex Human IL-10 2nd Gen Assay Cartridge
For research use only