Simple Plex Human IL-1 beta Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000216
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-1 beta Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 7 | 6 |
| Mean (pg/mL) | 8.66 | 446 | 8.42 | 410 |
| Standard Deviation | 0.427 | 16.5 | 0.342 | 23.3 |
| CV% | 4.9 | 3.7 | 4.0 | 5.7 |
Recovery for Simple Plex Human IL-1 beta Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 106 | 97-120 |
| Heparin Plasma (n=4) | 99 | 92-112 |
| Serum (n=4) | 105 | 96-112 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-1 beta Cartridge
Simple Plex Human IL-1 beta/IL-1F2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-1 beta/IL-1F2 Assay. Assay range is 0.4-1530 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-1 beta/IL-1F2 Products
Product Documents for Simple Plex Human IL-1 beta Cartridge
Product Specific Notices for Simple Plex Human IL-1 beta Cartridge
For research use only