Simple Plex Human G-CSF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002224
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human G-CSF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one G-CSF assay.
Inter-Assay Precision (Precision between assays) Replicates of each G-CSF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human G-CSF.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 58 | 58 |
| Mean (pg/mL) | 89.7 | 3851 | 84.8 | 3999 |
| Standard Deviation | 3.36 | 277 | 8.81 | 455 |
| CV% | 3.7 | 7.2 | 10.4 | 11.4 |
Recovery for Simple Plex Human G-CSF Cartridge
Recovery for the human G-CSF assays was evaluated at three different spiked concentrations of G-CSF protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 97 | 93-100 |
| Heparin Plasma (n=4) | 95 | 93-98 |
| Serum (n=4) | 101 | 98-106 |
Linearity
Samples containing and/or spiked with high concentrations of human G-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human G-CSF Cartridge
Simple Plex Human G-CSF Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human G-CSF Assay. Assay range is 3.13-11,936 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: G-CSF
G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
Long Name
Alternate Names
Gene Symbol
Additional G-CSF Products
Product Documents for Simple Plex Human G-CSF Cartridge
Product Specific Notices for Simple Plex Human G-CSF Cartridge
For research use only