Simple Plex Human Erythropoietin Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000487
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Erythropoietin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Epo assay.
Inter-Assay Precision (Precision between assays) Replicates of each Epo control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Epo.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 9 | 8 |
| Mean (mIU/mL) | 2.65 | 142 | 2.75 | 150 |
| Standard Deviation | 0.096 | 5.62 | 0.213 | 9.44 |
| CV% | 3.6 | 4.0 | 7.8 | 6.3 |
Recovery for Simple Plex Human Erythropoietin Cartridge
Recovery for the human Epo assays was evaluated at three different spiked concentrations of Epo protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 108 | 106-111 |
| EDTA Plasma (n=4) | 115 | 97-125 |
| Heparin Plasma (n=4) | 113 | 97-129 |
| Serum (n=4) | 113 | 111-115 |
Linearity
Samples containing and/or spiked with high concentrations of human EPO were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Erythropoietin Cartridge
Simple Plex Human Epo Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Epo Assay. Assay range is 0.36-550 mIU/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Erythropoietin/EPO
Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.
Alternate Names
Gene Symbol
Additional Erythropoietin/EPO Products
Product Documents for Simple Plex Human Erythropoietin Cartridge
Product Specific Notices for Simple Plex Human Erythropoietin Cartridge
For research use only