Simple Plex Human CXCL12/SDF-1 alpha Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000299
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 41 |
| Mean (pg/mL) | 27.1 | 1221 | 22.7 | 1049 |
| Standard Deviation | 1.56 | 66.5 | 3.05 | 77.4 |
| CV% | 5.8 | 5.4 | 13.4 | 7.4 |
Recovery for Simple Plex Human CXCL12/SDF-1 alpha Cartridge
Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CXCL12/SDF-1 alpha Cartridge
Human SDF-1 alpha Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CXCL12/SDF-1 alpha
CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.
Alternate Names
Gene Symbol
Additional CXCL12/SDF-1 alpha Products
Product Documents for Simple Plex Human CXCL12/SDF-1 alpha Cartridge
Product Specific Notices for Simple Plex Human CXCL12/SDF-1 alpha Cartridge
For research use only