Simple Plex Human CTLA-4 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003114
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CTLA-4 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CTLA-4 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CTLA-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CTLA-4.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 54 | 51 |
| Mean (pg/mL) | 209 | 9821 | 212 | 9517 |
| Standard Deviation | 8.97 | 616 | 16.4 | 701 |
| CV% | 4.3 | 6.3 | 7.7 | 7.4 |
Recovery for Simple Plex Human CTLA-4 Cartridge
Recovery for the human CTLA-4 assays was evaluated at three different spiked concentrations of CTLA-4 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 89 | 83-99 |
| Heparin Plasma (n=4) | 87 | 84-90 |
| Serum (n=4) | 95 | 89-100 |
Linearity
Samples containing and/or spiked with high concentrations of human CTLA-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human CTLA-4 Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CTLA-4 Assay. Assay range is 3.14-30,000 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: CTLA-4
CTLA-4/CD152 (Cytotoxic T-Lymphocyte Antigen 4) is a transmembrane T cell inhibitory molecule that is expressed as a covalent homodimer. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending CD28-dependent T cell activation. Mice deleted for CTLA-4 develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells which constitutively express CTLA-4.
Long Name
Alternate Names
Gene Symbol
Additional CTLA-4 Products
Product Documents for Simple Plex Human CTLA-4 Cartridge
Product Specific Notices for Simple Plex Human CTLA-4 Cartridge
For research use only