Simple Plex Human CCL22/MDC Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000866
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL22/MDC Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CCL22/MDC assay.
Inter-Assay Precision (Precision between assays) Replicates of each CCL22/MDC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CCL22/MDC.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 17 |
| Mean (pg/mL) | 17.2 | 975 | 17.5 | 890 |
| Standard Deviation | 0.947 | 68.6 | 1.23 | 49.9 |
| CV% | 5.5 | 7.0 | 7.0 | 5.6 |
Recovery for Simple Plex Human CCL22/MDC Cartridge
Recovery for the human CCL22/MDC assays was evaluated at three different spiked concentrations of CCL22/MDC protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 102 | 87-108 |
Linearity
Samples containing and/or spiked with high concentrations of human CCL22/MDC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL22/MDC Cartridge
Simple Plex Human CCL22/MDC Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CCL22/MDC Assay. Assay range is 0.68-2600 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL22/MDC
Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.
Additional CCL22/MDC Products
Product Documents for Simple Plex Human CCL22/MDC Cartridge
Product Specific Notices for Simple Plex Human CCL22/MDC Cartridge
For research use only