Simple Plex Human C-Reactive Protein/CRP Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000200
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human C-Reactive Protein/CRP Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CRP assay.
Inter-Assay Precision (Precision between assays) Replicates of each CRP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CRP.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 22 |
| Mean (pg/mL) | 291 | 18310 | 282 | 18318 |
| Standard Deviation | 19.6 | 783 | 14.2 | 1170 |
| CV% | 6.7 | 4.3 | 5.0 | 6.4 |
Recovery for Simple Plex Human C-Reactive Protein/CRP Cartridge
Recovery for the human CRP assays was evaluated at three different spiked concentrations of CD40 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=3) | 99 | 97-101 |
Linearity
<div>Samples containing and/or spiked with high concentrations of human CRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.</div>
Scientific Data Images for Simple Plex Human C-Reactive Protein/CRP Cartridge
Simple Plex Human CRP Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CRP Assay. Assay range is 32.8-50,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
Additional C-Reactive Protein/CRP Products
Product Documents for Simple Plex Human C-Reactive Protein/CRP Cartridge
Product Specific Notices for Simple Plex Human C-Reactive Protein/CRP Cartridge
For research use only