Simple Plex Human BDNF Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000389
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human BDNF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BDNF assay.
Inter-Assay Precision (Precision between assays) Replicates of each BDNF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BDNF.
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 20 | 22 |
| Mean (pg/mL) | 207 | 10,079 | 219 | 11,200 |
| Standard Deviation | 19.8 | 867 | 22.9 | 1144 |
| CV% | 9.6 | 8.6 | 10.5 | 10.2 |
Recovery for Simple Plex Human BDNF Cartridge
Recovery for the human BDNF assays was evaluated at three different spiked concentrations of BDNF protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Platelet-poor EDTA Plasma (n=4) | 82 | 76-86 |
Linearity
Samples containing and/or spiked with high concentrations of human free BDNF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human BDNF Cartridge
Simple Plex Human BDNF Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BDNF Assay. Assay range is 9.44-36,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Long Name
Alternate Names
Gene Symbol
Additional BDNF Products
Product Documents for Simple Plex Human BDNF Cartridge
Product Specific Notices for Simple Plex Human BDNF Cartridge
For research use only