Simple Plex Human CCL3/MIP-1 alpha Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000476
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL3/MIP-1 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MIP-1 alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each MIP-1 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MIP-1 alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 15 | 15 |
| Mean (pg/mL) | 22.5 | 1222 | 21.8 | 1179 |
| Standard Deviation | 1.05 | 58.7 | 0.910 | 46.1 |
| CV% | 4.7 | 4.8 | 4.2 | 3.9 |
Recovery for Simple Plex Human CCL3/MIP-1 alpha Cartridge
Recovery for the human MIP-1 alpha assays was evaluated at three different spiked concentrations of MIP-1 alpha protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 94 | 89-99 |
| Heparin Plasma (n=4) | 93 | 90-99 |
| Serum (n=4) | 97 | 93-100 |
Linearity
Samples containing and/or spiked with high concentrations of MIP-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human CCL3/MIP-1 alpha Cartridge
Simple Plex Human MIP-1 alpha Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL3/MIP-1 alpha
CCL3/MIP-1 alpha, is a beta chemokine that can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells. It forms complexes with sulfated proteoglycans and also associates into noncovalently-linked dimers and high molecular weight polymers. These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine. CCL3 signals through CCR1, CCR3, and CCR5, and it is internalized by the decoy chemokine receptor D6. CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types. It can promote tumor metastasis, hyperalgesia, and inflammation and suppress HIV replication and hematopoietic stem cell proliferation.
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional CCL3/MIP-1 alpha Products
Product Documents for Simple Plex Human CCL3/MIP-1 alpha Cartridge
Product Specific Notices for Simple Plex Human CCL3/MIP-1 alpha Cartridge
For research use only