Simple Plex HIV-1 Gag p24 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000981
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex HIV-1 Gag p24 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 35 | 34 |
| Mean (pg/mL) | 105 | 4500 | 94.3 | 4074 |
| Standard Deviation | .37 | 237 | 9.64 | 377 |
| CV% | 7.1 | 5.3 | 10.2 | 9.3 |
Recovery for Simple Plex HIV-1 Gag p24 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 114 | 111-118 |
| EDTA Plasma (n=4) | 107 | 102-116 |
| Heparin Plasma (n=4) | 107 | 99-112 |
| Serum (n=4) | 111 | 109-113 |
Linearity
Samples containing and/or spiked with high concentrations of human HIV Gag p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex HIV-1 Gag p24 Cartridge
HIV Gag p24 Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional HIV-1 Gag p24 Products
Product Documents for Simple Plex HIV-1 Gag p24 Cartridge
Product Specific Notices for Simple Plex HIV-1 Gag p24 Cartridge
For research use only