Simple Plex Discovery Human TGF-alpha Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012306
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human TGF-alpha Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 42 | 42 |
| Mean (pg/mL) | 6.03 | 283 | 5.47 | 261 |
| Standard Deviation | 0.174 | 6.18 | 0.522 | 17.9 |
| CV% | 2.9 | 2.2 | 9.5 | 6.9 |
Recovery for Simple Plex Discovery Human TGF-alpha Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 97 | 97-98 |
| EDTA Plasma (n=3) | 96 | 91-101 |
| Heparin Plasma (n=2) | 99 | 98-100 |
| Serum (n=2) | 98 | 94-102 |
Linearity
Samples containing and/or spiked with high concentrations of human TGF-alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human TGF-alpha Assay
Simple Plex Discovery Human TGF-alpha Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: TGF-alpha
TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional TGF-alpha Products
Product Documents for Simple Plex Discovery Human TGF-alpha Assay
Product Specific Notices for Simple Plex Discovery Human TGF-alpha Assay
For research use only