Simple Plex Discovery Human Serpin F1/PEDF Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015287
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Serpin F1/PEDF Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Serpin F1/PEDF assay.
Inter-Assay Precision (Precision between assays) Replicates of each Serpin F1/PEDF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Serpin F1/PEDF.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 85.4 | 4354 | 97.8 | 4477 |
| Standard Deviation | 2.96 | 157 | 7.23 | 190 |
| CV% | 3.5 | 3.6 | 7.4 | 4.3 |
Recovery for Simple Plex Discovery Human Serpin F1/PEDF Assay
Recovery for the human Serpin F1/PEDF assays was evaluated at three different spiked concentrations of Serpin F1/PEDF protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 112 | 111-113 |
Linearity
Samples containing and/or spiked with high concentrations of Serpin F1/PEDF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Serpin F1/PEDF Assay
Simple Plex Human Serpin F1/PEDF Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Serpin F1/PEDF Assay. Assay range is 7.86-12,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Serpin F1/PEDF
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Long Name
Alternate Names
Gene Symbol
Additional Serpin F1/PEDF Products
Product Documents for Simple Plex Discovery Human Serpin F1/PEDF Assay
Product Specific Notices for Simple Plex Discovery Human Serpin F1/PEDF Assay
For research use only