Simple Plex Discovery Human Pro-Collagen I alpha 1

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015353

Simple Plex Human Pro-Collagen Ia1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

3 pg/mL

Assay Range

26.4-40348 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Pro-Collagen I alpha 1 Summary

Simple Plex Discovery assay is for the detection of human Pro-Collagen I alpha 1 (Pro-Collagen I alpha1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Pro-Collagen I alpha 1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human Pro-Collagen I alpha1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Pro-Collagen I alpha1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Pro-Collagen I alpha1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Pro-Collagen I alpha1.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 18 18
Mean (pg/mL) 339 16608 346 16281
Standard Deviation 15.1 403 40.0 1256
CV% 4.4 2.4 11.6 7.7

Recovery for Simple Plex Discovery Human Pro-Collagen I alpha 1

Recovery for the human Pro-Collagen I alpha1 assays was evaluated at three different spiked concentrations of Pro-Collagen I alpha1 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 96 95-98

Linearity

Samples containing and/or spiked with high concentrations of Pro-Collagen I alpha1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Pro-Collagen I alpha1 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Pro-Collagen I alpha 1

Simple Plex Discovery Human Pro-Collagen I alpha1 Assay Standard Curve

Simple Plex Discovery Human Pro-Collagen I alpha1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Pro-Collagen I alpha1 Assay. Assay range is 26.4-40.348 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pro-Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Long Name

Pro-Collagen I alpha 1

Additional Pro-Collagen I alpha 1 Products

Product Documents for Simple Plex Discovery Human Pro-Collagen I alpha 1

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human Pro-Collagen I alpha 1

For research use only

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