Simple Plex Discovery Human Park7/DJ-1 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012424
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Park7/DJ-1 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 33 | 33 |
| Mean (pg/mL) | 141 | 6963 | 153 | 7341 |
| Standard Deviation | 8.65 | 411 | 11.9 | 486 |
| CV% | 6.1 | 5.9 | 7.8 | 6.6 |
Recovery for Simple Plex Discovery Human Park7/DJ-1 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 98 | 91-105 |
Linearity
Samples containing and/or spiked with high concentrations of Park7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Park7/DJ-1 Assay
Simple Plex Discovery Human Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Park7/DJ-1
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.
Long Name
Alternate Names
Gene Symbol
Additional Park7/DJ-1 Products
Product Documents for Simple Plex Discovery Human Park7/DJ-1 Assay
Product Specific Notices for Simple Plex Discovery Human Park7/DJ-1 Assay
For research use only